Quantitative interactome analysis with chemical cross-linking and mass spectrometry. (February 2022)

• Record Type: Journal Article
• Authors: Wippel, Helisa H.; Chavez, Juan D.; Tang, Xiaoting; Bruce, James E.
• Is Part Of: Current opinion in chemical biology. Volume 66(2022)
• Publication Date: 2022-02
• Subjects: Quantitative cross-linking -- Protein interaction -- Protein conformation -- Mass spectrometry -- Quantitative interactomics; BS3 bis(sulfosuccinimidyl)suberate -- CBDPS CyanurBiotinDimercaptoPropionylSuccinimide -- DSBU disuccinimidyl dibutryic urea -- DSS disuccinimidyl suberate -- iqPIR isobaric quantitative protein interaction reporter -- LC-MS liquid chromatography – mass spectrometry -- MS mass spectrometry -- NHP N-hyrdoxyphthalimide -- PIR protein interaction reporter -- PRM parallel reaction monitoring -- PTX paclitaxel -- SILAC stable isotope labeling by amino acids in cell culture -- TMT tandem mass tags -- qXL-MS quantitative chemical cross-linking with mass spectrometry; Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2021.06.011
• Languages: English
• ISSNs: 1367-5931

Abstract

Abstract: Structural plasticity and dynamic protein–protein interactions are critical determinants of protein function within living systems. Quantitative chemical cross-linking with mass spectrometry (qXL-MS) is an emerging technology able to provide information on changes in protein conformations and interactions. Importantly, qXL-MS is applicable to complex biological systems, including living cells and tissues, thereby providing insights into proteins within their native environments. Here, we present an overview of recent technological developments and applications involving qXL-MS, including design and synthesis of isotope-labeled cross-linkers, development of new liquid chromatography–MS methodologies, and computational developments enabling interpretation of the data. Graphical abstract: Image 1 Highlights: Quantitative chemical cross-linking provides information on changes in protein interactomics. Overview of recent technological developments involving qXL-MS. Applications of qXL-MS in biological systems with varying conditions.