Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron‐transfer, Carbohydrate‐mimetic/Cross‐linker‐peptide‐collagen Film. Issue 3 (17th November 2021)

• Record Type: Journal Article
• Authors: Ishizaki, Sora; Kuramitz, Hideki; Sugawara, Kazuharu
• Is Part Of: Electroanalysis. Volume 34:Issue 3(2022)
• Page Start: 464
• Page End: 473
• Publication Date: 2021-11-17
• Subjects: Electron-transfer peptide -- Carbohydrate-mimetic peptide -- Soybean agglutinin -- Tyrosine -- Collagen; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202100380
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: We constructed an electrochemical sensor based on an electrode modified with electron‐transfer, carbohydrate‐mimetic peptides on collagen film. The peptide consisted of Ac‐Y4 C combined with soybean agglutinin (SBA). To evaluate the binding between SBA and the peptide, we prepared Ac‐Y4 CAn (3‐6) containing oligoalanine as a cross‐linker. When SBA and Y4 C on the electrode were incubated, the peak was decreased by the SBA uptake of the peptide. The change in the peak current using Ac‐Y4 CA6 was the greatest of the four peptides. The calibration curve was linear and ranged from 4.0×10 −14 to 1.2×10 −12 M with a detection limit of 1.3×10 −14 M. Abstract :