Enhanced production of nonanedioic acid from nonanoic acid by engineered Escherichia coli. Issue 3 (28th May 2021)

• Record Type: Journal Article
• Authors: Lee, Yongjoo; Sathesh‐Prabu, Chandran; Kwak, Geun Hwa; Bang, Ina; Jung, Hyun Wook; Kim, Donghyuk; Lee, Sung Kuk
• Is Part Of: Biotechnology journal. Volume 17:Issue 3(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-28
• Subjects: adaptive laboratory evolution -- biotransformation -- medium chain fatty acids -- nonanedioic acid -- nonanoic acid; Biotechnology -- Periodicals; 660.605
• DOI: 10.1002/biot.202000416
• Languages: English
• ISSNs: 1860-6768

Abstract

Abstract: In this study, whole‐cell biotransformation was conducted to produce nonanedioic acid from nonanoic acid by expressing the alkane hydroxylating system (AlkBGT) from Pseudomonas putida GPo1 in Escherichia coli . Following adaptive laboratory evolution, an efficient E . coli mutant strain, designated as MRE, was successfully obtained, demonstrating the fastest growth (27‐fold higher) on nonanoic acid as the sole carbon source compared to the wild‐type strain. Additionally, the MRE strain was engineered to block nonanoic acid degradation by deleting fadE . The resulting strain exhibited a 12.8‐fold increase in nonanedioic acid production compared to the wild‐type strain. Six mutations in acrR, P crp, dppA, P fadD, e14, and yeaR were identified in the mutant MRE strain, which was characterized using genomic modifications and RNA‐sequencing. The acquired mutations were found to be beneficial for rapid growth and nonanedioic acid production. Graphical Abstract and Lay Summary: Wild‐type E. coli strains do not grow properly when using nonanoic acid as the sole source of carbon. After adaptive laboratory evolution, a fast‐growing mutant on nonanoic acid was obtained. The evolved strain can efficiently produce nonanedioic acid from nonanoic acid by expressing the ω‐oxidation pathway and additional genetic modification.