Shedding Light on DNA‐Based Nanoprobes for Live‐Cell MicroRNA Imaging. Issue 10 (2nd December 2021)

• Record Type: Journal Article
• Authors: Yang, Fan; Lu, Huiting; Meng, Xiangdan; Dong, Haifeng; Zhang, Xueji
• Is Part Of: Small. Volume 18:Issue 10(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-02
• Subjects: biosensors -- delivery vehicles -- DNA‐based nanoprobes -- in situ detection -- microRNA imaging; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202106281
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: DNA‐based nanoprobes integrated with various imaging signals have been employed for fabricating versatile biosensor platforms for the study of intracellular biological process and biomarker detection. The nanoprobes developments also provide opportunities for endogenous microRNA (miRNA) in situ analysis. In this review, the authors are primarily interested in various DNA‐based nanoprobes for miRNA biosensors and declare strategies to reveal how to customize the desired nanoplatforms. Initially, various delivery vehicles for nanoprobe architectures transmembrane transport are delineated, and their biosecurity and ability for resisting the complex cellular environment are evaluated. Then, the novel strategies for designing DNA sequences as target miRNA specific recognition and signal amplification modules for miRNA detection are presented. Afterward, recent advances in imaging technologies to accurately respond and produce significant signal output are summarized. Finally, the challenges and future directions in the field are discussed. Abstract : DNA‐based nanoprobes have recently emerged as a reliable detection tool for microRNA imaging, which provides spatial‐temporal and dynamic physiological or pathological information. In this review, various nanoprobes from carrier selections, sequence designs, and imaging technologies are introduced, aiming to reveal how to construct novel nanoprobes with desired performance, which is a benefit for biomedical application and precision medicine.