Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis. (10th May 2022)

• Record Type: Journal Article
• Authors: Wang, Rongchun; Ren, Qingyu; Gao, Daili; Paudel, Yam Nath; Li, Xia; Wang, Lizhen; Zhang, Pengyu; Wang, Baokun; Shang, Xueliang; Jin, Meng
• Is Part Of: Journal of ethnopharmacology. Volume 289(2022)
• Publication Date: 2022-05-10
• Subjects: Neuropsychic diseases -- Network pharmacology -- PC12 -- Anti-depressant activity -- Zebrafish; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2022.115018
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Gastrodia elata Blume ( G. elata ), a traditional Chinese herb, known as "Tian Ma", is widely used as a common medicine and diet ingredient for treating or preventing neurological disorders for thousands of years in China. However, the anti-depressant effect of G. elata and the underlying mechanism have not been fully evaluated. Aim of the study: The study is aimed to investigate the anti-depressant effect and the molecular mechanism of G. elata in vitro and in vivo using PC12 cells and zebrafish model, respectively. Material and methods: Network pharmacology was performed to explore the potential active ingredients and action targets of G. elata Blume extracts (GBE) against depression. The cell viability and proliferation were determined by MTT and EdU assay, respectively. TUNEL assay was used to examine the anti-apoptotic effect of GBE. Immunofluorescence and Western blot were used to detect the protein expression level. In addition, novel tank diving test was used to investigate the anti-depressant effect in zebrafish depression model. RT-PCR was used to analyze the mRNA expression levels of genes. Results: G. elata against depression on the reticulon 4 receptors (RTN4R) and apoptosis-related targets, which were predicted by network pharmacology. Furthermore, GBE enhanced cell viability and inhibited the apoptosis in PC12 cells against CORT treatment. GBE relieved depression-like symptoms in adult zebrafish, included increase of exploratory behavior and regulation of depression related genes. Mechanism studies showed that the GBE inhibited the expression of RTN4R-related and apoptosis-related genes. Conclusion: Our studies show the ameliorative effect of G. elata against depression. The mechanism may be associated with the inhibition of RTN4R-related and apoptosis pathways. Graphical abstract: Image 1 Highlights: Network pharmacology was used to reveal the anti-depressant activity of G. elata Blume extracts (GBE) and its pharmacological mechanism. GBE suppressed depression-like symptoms in zebrafish and cellular depression models. Ameliorative effect of GBE on depression is associated with the inhibition of RTN4R-related and apoptosis pathways.