A ligand-based ELISA for detection of soluble asialoglycoprotein receptor in human serum. Issue 7 (22nd November 2021)

• Record Type: Journal Article
• Authors: Hu, Jing; Zhao, Ming; Shi, Qimin; Li, Lingxin; Yin, Jian
• Is Part Of: Journal of carbohydrate chemistry. Volume 40:Issue 7/9(2021)
• Page Start: 440
• Page End: 453
• Publication Date: 2021-11-22
• Subjects: Asialoglycoprotein receptor (ASGPR) -- ELISA -- human serum -- ligand -- liver function; Carbohydrates -- Periodicals; 547.78
• DOI: 10.1080/07328303.2021.2008952
• Languages: English
• ISSNs: 0732-8303

Abstract

Abstract: A ligand-based enzyme-linked immunosorbent assay (ELISA) for the soluble form of functional asialoglycaoprotein receptor (s-ASGPR) in human serum has been developed. This assay was evaluated and proved to show good stability, which is suitable for testing human serum. Clinical serum samples were collected and tested with the established assay. The contents of serum s-ASGPR in healthy group and liver injury group were compared ( p < 0.001). The functional s-ASGPR can be a promising candidate of noninvasive biomarker for liver function and injury. The receiver-operating characteristic (ROC) curve analysis illustrated that this economical, sensitive, and reproducible quantitative assay has great potential for clinical use. Graphical Abstract: UF0001