A specific esterase and pH logically regulate ESIPT: different kinds of granulocyte sorting. Issue 17 (8th February 2022) - Wang, Kui
Abstract : The ESIPT process of HBT-ASDs was synergistically activated by dual-factors, NAS-DCE and pH. They emit logical and differential fluorescence response signals to sort the acid, neutrophil and alkaline granulocytes. Abstract : Simultaneously detecting naphthol AS-D chloroacetate esterase (NAS-DCE ) and pH is an effective way to separate different granulocytes, which is of great significance for the analysis of blood. A series of fluorescent small molecules (HBT-ASDs ) were designed, whose ESIPT process could be logically regulated by NAS-DCE and pH. One typical molecule, HBT-ASD-2, emits three kinds of fluorescence output signal at 438 nm and 545 nm for NAS-DCE under different pH values (5.0, 7.4 and 10, respectively). According to such differential signals, the acid, neutrophil and alkaline granulocytes can be sorted, and the activity of NAS-DCE can also be simultaneously monitored in real-time. Thus, a simple analytical tool for clinical blood monitoring and analysis is provided.