Application of scanning electrochemical microscopy for topography imaging of supported lipid bilayers. Issue 10 (24th February 2022)

• Record Type: Journal Article
• Authors: Nasri, Zahra; Memari, Seyedali; Striesow, Johanna; Weltmann, Klaus-Dieter; von Woedtke, Thomas; Wende, Kristian
• Is Part Of: Analytical methods. Volume 14:Issue 10(2022)
• Page Start: 1077
• Page End: 1082
• Publication Date: 2022-02-24
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d2ay00154c
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : The present study demonstrates the application of scanning electrochemical microscopy to follow the morphological changes of the lipid bilayer under oxidative stress or due to the action of membrane proteins or antimicrobial peptides. Abstract : Oxidative stress in cellular environments may cause lipid oxidation and membrane degradation. Therefore, studying the degree of lipid membrane morphological changes by reactive oxygen and nitrogen species will be informative in oxidative stress-based therapies. This study introduces the possibility of using scanning electrochemical microscopy as a powerful imaging technique to follow the topographical changes of a solid-supported lipid bilayer model induced by reactive species produced from gas plasma. The introduced strategy is not limited to investigating the effect of reactive species on the lipid bilayer but could be extended to understand the morphological changes of the lipid bilayer due to the action of membrane proteins or antimicrobial peptides.