Precise and long-term tracking of mitochondria in neurons using a bioconjugatable and photostable AIE luminogen. Issue 10 (21st February 2022)

• Record Type: Journal Article
• Authors: Park, Hojeong; Niu, Guangle; Wu, Chao; Park, Chungwon; Liu, Haixiang; Park, Hyokeun; Kwok, Ryan T. K.; Zhang, Jing; He, Benzhao; Tang, Ben Zhong
• Is Part Of: Chemical science. Volume 13:Issue 10(2022)
• Page Start: 2965
• Page End: 2970
• Publication Date: 2022-02-21
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1sc06336g
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : A novel bioconjugatable and photostable AIE luminogen has been rationally synthesized for precise and long-term tracking of neuron mitochondria. Abstract : Tracking mitochondrial movement in neurons is an attractive but challenging research field as dysregulation of mitochondrial motion is associated with multiple neurological diseases. To realize accurate and long-term tracking of mitochondria in neurons, we elaborately designed a novel aggregation-induced emission (AIE)-active luminogen, TPAP-C5-yne, where we selected a cationic pyridinium moiety to target mitochondria and employed an activated alkyne terminus to achieve long-term tracking through bioconjugation with amines on mitochondria. For the first time, we successfully achieved the accurate analysis of the motion of a single mitochondrion in live primary hippocampal neurons and the long-term tracking of mitochondria for up to a week in live neurons. Therefore, this new AIEgen can be used as a potential tool to study the transport of mitochondria in live neurons.