Pseudaquidulcibacter saccharophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source. Issue 2 (24th February 2022)

• Record Type: Journal Article
• Authors: Liu, Yaqi; Li, Shuyang; Lin, Jing; Liu, Shuangyuan; Wei, Hehong; Dai, Jingcheng; Qiu, Dongru
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 2(2022)
• Publication Date: 2022-02-24
• Subjects: starch -- effluent -- strain SZ9T -- Pseudaquidulcibacter saccharophilus; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005264
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9 T, was isolated from the effluent sample. Colonies of strain SZ9 T were small (approximately 0.5–1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex. Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative. Growth occurred at 10–40 °C (optimum, 28 °C) and pH 5.5–8.0 (optimum, pH 7.5). The range of NaCl concentration for growth was 0–1.0 % (w/v), with an optimum of 0–0.5 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9 T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2 T (92.44%), followed by Vitreimonas flagellata SYSU XM001 T (89.61 %), Asprobacter aquaticus DRW22-8 T (89.49 %) and Hyphobacterium vulgare WM6 T (89.49%). The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9 T was summed feature 3 (comprising C16 : 1 ω 6 c and/or C16 : 1 ω 7 c ), summed feature 8 (C18 : 1 ω 6 c and/or C18 : 1 ω 7 c ) and C16 : 0 . The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids. The whole genome of strain SZ9 T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%. The orthologous average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain SZ9 T and other genera within the family Caulobacteraceae were 64.50–66.62 %, 46.96–54.17 % and 27.70–31.70 %, respectively. Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9 T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp. nov is proposed. The type strain is SZ9 T (=CCTCC AB2021029 T =KCTC 82788 T ).