Co-degradation of ofloxacin and its impact on solid phase denitrification with polycaprolactone as carbon source. (April 2022)

• Record Type: Journal Article
• Authors: Xie, Yu; Wang, Pengbo; Li, Peng; He, Yiliang
• Is Part Of: Bioresource technology. Volume 350(2022)
• Publication Date: 2022-04
• Subjects: Solid-phase denitrification -- PCL -- Antibiotics -- Ofloxacin -- Co-degrade; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.126938
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Exposure to low concentration of ofloxacin would decrease denitrification rate. Ofloxacin could be co-degraded with polycaprolactone in solid phase denitrification. Selective pressure of OFX leads to biodiversity elimination of denitrifying sludge. Hydroxylation is found to be the critical step for biodegradation of OFX. ABC transporters and cytochrome P450 related to multi-drug resistance were expressed. Abstract: Solid-phase denitrification has been applied for advanced nitrogen removal from wastewater and can co-degrade emerging pollutants. Fluoroquinolones (FQs), broad-spectral antibiotic, are frequently detected in the effluent of conventional wastewater treatment plants. However, it remains unclear whether solid-phase denitrifying bacteria can remove FQs. Thus, this study investigated the removal capacity of ofloxacin (OFX) as a representative of FQs and the microbial community structures of denitrifying sludge acclimated to polycaprolactone and OFX. The Results indicate that OFX had a negative effect on denitrification performance. OFX was degraded, and a possible pathway was revealed based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The dominant genera in the acclimated denitrifying sludge were Microbacterium, Simplicispira, Alicycliphilus, Reyranella, Sediminibacterium, Acidovorax and Thermomonas . Moreover, ABC transporters and cytochrome P450, related to multi-drug resistance and drug metabolism, were highly expressed in the acclimated sludge. This study provides novel insights into antibiotics control.