An integrin alpha 4 (ChIntα 4) from oyster Crassostrea hongkongensis mediates the hemocytes phagocytosis towards Vibrio alginolyticus. Issue 122 (March 2022)

• Record Type: Journal Article
• Authors: Fu, Yao-Wu; Chen, Wei-Feng; He, Meng-Han; Tang, Lei; Guo, Shu-Quan; Zhang, Qi-Zhong
• Is Part Of: Fish & shellfish immunology. Issue 122(2022)
• Page Start: 246
• Page End: 256
• Publication Date: 2022-03
• Subjects: Crassostrea hongkongensis -- Integrin -- Agglutination -- Phagocytosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.02.015
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Integrins, a family of cell adhesion transmembrane receptors, mediate cell adhesion, migration, proliferation, apoptosis, and phagocytosis. In the present study, an integrin ChIntα 4 from Crassostrea hongkongensis was characterized to investigate its role in defensing against pathogenic bacterium Vibrio alginolyticus . The full-length cDNA sequence of ChIntα 4 was 3572 bp with an open reading frame (ORF) of 3168 bp, which encoded a polypeptide with 1055 amino acids. The mRNA expression of ChIntα 4 in the hemocytes was significantly up-regulated at 6 h and 24 h post V. alginolyticus stimulation ( p < 0.01). The recombinant Ch Intα 4 protein could agglutinate the rabbit red blood cells and Gram-negative bacteria V. alginolyticus and Escherichia coli . Moreover, the phagocytic activity of the hemocytes was significantly down-regulated from 46.9% to 32.7% when blocked with anti- Ch Intα 4 antibody, and it was significantly up-regulated from 42.7% to 59.5% post transfection with pCI-neo- ChIntα 4 plasmid ( p < 0.05). In conclusion, these findings demonstrated that Ch Intα 4 might be involved in resisting V. alginolyticus infection and regulating phagocytosis as a cell adhesion receptor in C. hongkongensis . Highlights: The sequence of ChIntα 4 was firstly cloned from Crassostrea hongkongensis. The mRNA level of ChIntα 4 showed positive response post Vibrio stimulation. Ch Intα 4 protein agglutinated the erythrocyte and Gram-negative bacteria. Ch Intα 4 protein was demonstrated to regulate phagocytosis of the hemocytes.