Involvement of increased endoplasmic reticulum stress in the development of cataracts in BALB.NCT-Cpoxnct mice. (February 2022)

• Record Type: Journal Article
• Authors: Liu, Chang; Miyahara, Hiroki; Dai, Jian; Cui, Xiaoran; Li, Ying; Kang, Xiaojing; Higuchi, Keiichi; Mori, Masayuki
• Is Part Of: Experimental eye research. Volume 215(2022)
• Publication Date: 2022-02
• Subjects: Cataract -- Endoplasmic reticulum stress -- Crystallin -- Mouse -- Coproporphyrin -- Protein aggregation -- Transcriptomics; Ophthalmology -- Periodicals; Eye -- Periodicals; Œil -- Périodiques; Ophthalmology; Periodicals; Electronic journals; 612.8405
• DOI: 10.1016/j.exer.2021.108905
• Languages: English
• ISSNs: 0014-4835

Abstract

Abstract: The BALB.NCT- Cpox nct is a mutant mouse model for hereditary cataracts. We previously uncovered that the primary cause of the cataracts of BALB.NCT- Cpox nct is a mutation in the coproporphyrinogen oxidase ( Cpox ) gene. Because of the mutation, excessive coproporphyrin is accumulated in the BALB.NCT- Cpox nct lens. In this study, we analyzed the changes in transcriptome and proteins in the lenses of 4- and 12-week-old BALB.NCT- Cpox nct to further elucidate the molecular etiology of cataracts in this mouse strain. Transcriptome analysis revealed that endoplasmic reticulum (ER) stress was increased in the BALB.NCT- Cpox nct lens that induced persistent activation of the PERK signaling pathway of the ER stress response. Also, levels of crystallin transcripts and proteins were reduced in the BALB.NCT- Cpox nct lens. Analysis of proteins disclosed aggregation of crystallins and keratins prior to the manifestation of cataracts in 4-week-old BALB.NCT- Cpox nct mice. At 12 weeks of age, insoluble crystallins were accumulated in the cataractous BALB.NCT- Cpox nct lens. Overall, our data suggest the following sequence of events in the BALB.NCT- Cpox nct lens: accumulated coproporphyrin induces the aggregation of proteins including crystallins. Aggregated proteins increase ER stress that, in turn, leads to the repression of global translation of proteins including crystallins. The decline in the molecular chaperone crystallin aggravates aggregation and insolubilization of proteins. This vicious cycle would eventually lead to cataracts in BALB.NCT- Cpox nct . Highlights: Coproporphyrin accumulation promotes endoplasmic reticulum stress in the mouse lens. Coproporphyrin induces aggregation of crystallins and keratins in the lens. Coproporphyrin accumulation reduces crystallin production in the lens. Lens homeostasis is stuck in a vicious cycle that eventually leads to cataracts.