GIRK channel as a versatile regulator of neurotransmitter release via L-type Ca2+ channel-dependent mechanism in the neuromuscular junction. (15th May 2022)

• Record Type: Journal Article
• Authors: Tsentsevitsky, Andrei N.; Khaziev, Eduard F.; Kovyazina, Irina V.; Petrov, Alexey M.
• Is Part Of: Neuropharmacology. Volume 209(2022)
• Publication Date: 2022-05-15
• Subjects: Neuromuscular junction -- Neurotransmitter release -- Voltage gated Ca2+ channels -- M2-AChR -- GIRK channels; ACh acetylcholine -- AP action potential -- NMJ neuromuscular junction -- EPC endplate current -- EPP endplate potential -- MEPC miniature endplate current -- MEPP miniature endplate potential -- GIRK channel G protein-gated inwardly rectifying potassium channel -- LTCC L-type Ca2+ channel -- M-AChR muscarinic ACh receptor -- ML-297 1-(3, 4-Difluorophenyl)-3-(3-methyl-1-phenyl-1H-pyrazol-5-yl)urea -- SV synaptic vesicle -- TPN-Q tertiapin-Q -- VGCC voltage-gated Ca2+channel; Neuropsychopharmacology -- Periodicals; Autonomic Agents -- Periodicals; Neuropsychopharmacologie -- Périodiques; Neuropsychopharmacology; Periodicals; Electronic journals; 615.78
• DOI: 10.1016/j.neuropharm.2022.109021
• Languages: English
• ISSNs: 0028-3908

Abstract

Abstract: G protein-gated inwardly rectifying potassium (GIRK) channels are one of the main regulators of neuronal excitability. Activation of GIRK channels in the CNS usually leads to postsynaptic inhibition. However, the function of GIRK channels in the presynaptic processes, notably neurotransmitter release form motor nerve terminals, is yet to be comprehensively understood. Here, using electrophysiological and fluorescent approaches, the role of GIRK channels in neurotransmitter release from frog motor nerve terminals was studied. We found that the inhibition of GIRK channels with nanomolar tertiapin-Q synchronized exocytosis events with action potential but suppressed spontaneous and evoked neurotransmitter release, as well as Ca 2+ transient and membrane permeability for K + . The action of GIRK channel inhibition on evoked neurotransmission was prevented by selective antagonist of voltage-gated Ca 2+ channels of L-type. Furthermore, the effects of muscarinic acetylcholine receptor activation on neurotransmitter release, Ca 2+ transient and K + channel activity were markedly modulated by inhibition of GIRK channels. Thus, at the motor nerve terminals GIRK channels can regulate timing of neurotransmitter release and be a positive modulator of synaptic vesicle exocytosis acting partially via L-type Ca 2+ channels. In addition, GIRK channels are key players in a feedback control of neurotransmitter release by muscarinic acetylcholine receptors. Graphical abstract: Image 1 Highlights: GIRK channels modulate intensity and timing of neurotransmitter release in NMJs. Inhibition of GIRK channels lead to suppression and synchronization of transmitter release. These effects can be result of decrease in LTCC activity and hence [Ca 2+ ]in transient. M2AChRs differentially regulate GIRK channel in [Ca 2+ ]out -dependent manner at NMJs.