Chitin/corn stalk pith sponge stimulated hemostasis with erythrocyte absorption, platelet activation, and Ca2+-binding capabilities. (15th May 2022)

• Record Type: Journal Article
• Authors: Cheng, Hao; Pan, Xin; Shi, Zhe; Huang, Xusheng; Zhong, Qiang; Liu, Haibing; Chen, Yuhang; Lian, Qiang; Wang, Jian; Shi, Zhanjun
• Is Part Of: Carbohydrate polymers. Volume 284(2022)
• Publication Date: 2022-05-15
• Subjects: BCI blood clotting index -- CSP corn stalk pith -- FBS fetal bovine serum -- PBS phosphate-buffered saline -- PRP platelet-rich plasma -- RBC red blood cells -- SD Sprague Dawley -- SEM scanning electron microscopy -- XRD X-ray diffractometer -- FT-IR Fourier-transform infrared spectroscopy -- PVF poly(vinyl formaldehyde); Chitin-corn stalk pith composite sponge -- Ball milling -- Hemocyte adsorption/aggregation -- Platelet activation -- Calcium ion binding; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118953
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Chitin (CT) is widely used as a hemostatic material in surgical sponges, although its efficacy needs improvement to promote the clotting process. In this study, another green biomass, corn stalk pith (CSP), was incorporated into CT through ball milling to fabricate CT-CSP composite hemostatic sponges to enhance erythrocyte absorption, platelet activation, and clotting factor accumulation (Ca 2+ ). In vitro hemostatic analysis indicated that CSP incorporation greatly promoted the coagulation process, with a much lower blood clot index and higher blood clot stability. In addition, the composite sponge promoted more platelet adhesion and activation, and the composite sponge demonstrated a greater ability to bind clotting factors (Ca 2+ ). Consistently, it achieved complete hemostasis with less blood loss and a shorter hemostatic time in a rat liver injury-model. This composite hemostatic sponge is sustainable, cost-efficient, and biocompatible, which highlight the excellent translational potential in clinical settings. Graphical abstract: Unlabelled Image