Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp). Issue 4 (April 2022)

• Record Type: Journal Article
• Authors: Suzuki, Masahiro; Norizuki, Chihiro; Wachino, Jun-ichi; Kawamura, Kumiko; Nagano, Noriyuki; Nagano, Yukiko; Hayashi, Wataru; Kimura, Kouji; Doi, Yohei; Arakawa, Yoshichika
• Is Part Of: Journal of infection and chemotherapy. Volume 28:Issue 4(2022)
• Page Start: 473
• Page End: 479
• Publication Date: 2022-04
• Subjects: I1 plasmid -- blaCTX-M -- OSNAp; OSNAp ORF-based binarized structure network analysis of plasmids -- pST plasmid sequence types -- pMLST plasmid multilocus sequence typing -- ST sequence type -- MLST multilocus sequence typing; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2021.12.003
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: Objectives: We aimed to elucidate the relationship among bla CTX-M -carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring bla CTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying bla CTX-M-1 or bla CTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying bla CTX-M-14 or bla CTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying bla CTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.