The dilemma of identifying Peptoniphilus species by using two MALDI-TOF MS systems. (February 2022)

• Record Type: Journal Article
• Authors: Barberis, Claudia; Litterio, Mirta; Venuta, María Elena; Maldonado, María Laura; Abel, Sofía; Fernández-Canigia, Liliana; Vaustat, Daniela; Azula, Natalia; Castello, Liliana; Legaria, María Cristina; Pereyra, Ana; Rossetti, Adelaida; Predari, Silvia Carla; Rollet, Raquel; Cejas, Daniela
• Is Part Of: Anaerobe. Volume 73(2022)
• Publication Date: 2022-02
• Subjects: Peptoniphilus spp. -- Identification -- MALDI-TOF MS -- Argentina; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2021.102500
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Two commercial MALDI-TOF MS systems were used to identify 18 isolates, belonging to the Peptoniphilus genus; also the 16S rRNA sequencing identity was compared against the MALDI-TOF MS system results. Bruker Biotyper system provided higher accuracy than Vitek MS system, however, adding spectra could allow a more reliable species level identification. Highlights: Discrepancies in the identification of Peptoniphilus by MALDI-TOF MS were studied. Incorrectly assigned reference strains in database can lead to misidentification. Two closely species could be reported as a complex by Bruker MALDI-TOF MS. Spectral databases should be updated by suppliers to improve identification rates.