Kinsenoside attenuates liver fibro-inflammation by suppressing dendritic cells via the PI3K-AKT-FoxO1 pathway. (March 2022)

• Record Type: Journal Article
• Authors: Xiang, Ming; Liu, Tingting; Tian, Cheng; Ma, Kun; Gou, Jing; Huang, Rongrong; Li, Senlin; Li, Qing; Xu, Chuanrui; Li, Lei; Lee, Chih-Hao; Zhang, Yonghui
• Is Part Of: Pharmacological research. Volume 177(2022)
• Publication Date: 2022-03
• Subjects: AKT protein kinase B -- BMDC bone marrow-derived debdritic cell -- DC dendritic cell -- ECM extracellular matrix -- FoxO1 forkhead box protein O1 -- HSC hepatic stellate cell -- IL-12 interleukin-12 -- KD kinsenoside -- LF liver fibrosis -- LPS lipopolysaccharide -- MFB myofibroblast -- PD-L1 programmed cell death ligand 1 -- PI3K phosphatidylinositol 3 kinase; Kinsenoside (PubChem CID:10422896) -- LY294002 (PubChem CID:3973) -- 740 Y-P (PubChem CID:90488730); Liver fibro-inflammation -- Kinsenoside -- Dendritic cell -- PI3K-AKT-FoxO1 axis -- Programmed cell death ligand 1 -- Interleukin-12; Pharmacology -- Periodicals; Pharmacology -- Periodicals; Research -- Periodicals; Médicaments -- Recherche -- Périodiques; Pharmacologie -- Périodiques; 615.105
• DOI: 10.1016/j.phrs.2022.106092
• Languages: English
• ISSNs: 1043-6618

Abstract

Abstract: Kinsenoside (KD) exhibits anti-inflammatory and immunosuppressive effects. Dendritic cells (DCs) are critical regulators of the pathologic inflammatory milieu in liver fibrosis (LF). Herein, we explored whether and how KD repressed development of LF via DC regulation and verified the pathway involved in the process. Given our analysis, both KD and adoptive transfer of KD-conditioned DCs conspicuously reduced hepatic histopathological damage, proinflammatory cytokine release and extracellular matrix deposition in CCl4 -induced LF mice. Of note, KD restrained the LF-driven rise in CD86, MHC-II, and CCR7 levels and, simultaneously, upregulated PD-L1 expression on DCs specifically, which blocked CD8 + T cell activation. Additionally, KD reduced DC glycolysis, maintained DCs immature, accompanied by IL-12 decrease in DCs. Inhibiting DC function by KD disturbed the communication of DCs and HSCs with the expression or secretion of α-SMA and Col-I declined in the liver. Mechanistically, KD suppressed the phosphorylation of PI3K-AKT driven by LF or PI3K agonist, followed by enhanced nuclear transport of FoxO1 and upregulated interaction of FoxO1 with the PD-L1 promoter in DCs. PI3K inhibitor or si-IL-12 acting on DC could relieve LF, HSC activation and diminish the effect of KD. In conclusion, KD suppressed DC maturation with promoted PD-L1 expression via PI3K-AKT-FoxO1 and decreased IL-12 secretion, which blocked activation of CD8 + T cells and HSCs, thereby alleviating liver injury and fibro-inflammation in LF. Graphical Abstract: ga1 Highlights: KD alleviated fibro-inflammation in LF by reprograming DC function via PI3K-AKT axis. KD enhanced FoxO1 binding to PD-L1 promoter in DC, inhibiting CD8 + T cell responses. KD decreased IL-12 secretion in DC to remit inflammation and block HSC activation.