Gut microbiota and its role in stress-induced hyperalgesia: Gender-specific responses linked to different changes in serum metabolites. (March 2022)

• Record Type: Journal Article
• Authors: Xu, Xiaolin; Zhan, Gaofeng; Chen, Rongmin; Wang, Danning; Guan, Shaodi; Xu, Hui
• Is Part Of: Pharmacological research. Volume 177(2022)
• Publication Date: 2022-03
• Subjects: Ampicillin sodium (PubChem CID: 23663979), Metronidazole (PubChem CID: 4173), Neomycin sulfate (PubChem CID: 24978553); CI confidence interval -- CRS chronic restraint stress -- ESI electrospray ionization -- FMT fecal microbiota transplantation -- KEGG kyoto encyclopedia of genes and genomes -- LC-MS liquid chromatography-mass spectrometry -- OPLS-DA orthogonal projections to latent structures-discriminate analysis -- PCoA principal coordinates analysis -- PWMT paw withdrawal mechanical threshold -- SIH stress-induced hyperalgesia -- TFL tail-flick latency -- TFT tail-flick test -- VIP variable importance projection; Chronic restraint stress -- Stress-induced hyperalgesia -- Gut microbiota -- Metabolomics -- Gender difference; Pharmacology -- Periodicals; Pharmacology -- Periodicals; Research -- Periodicals; Médicaments -- Recherche -- Périodiques; Pharmacologie -- Périodiques; 615.105
• DOI: 10.1016/j.phrs.2022.106129
• Languages: English
• ISSNs: 1043-6618

Abstract

Abstract: Long-term stress causes hyperalgesia; and there are gender differences in the mechanism of pain in male and female individuals. The role of gut microbiota in pain has also been verified. However, whether gut microbiota plays a role in hyperalgesia caused by chronic restraint stress (CRS) with gender differences has not been explored. This study investigated the role of gut microbiota in CRS-induced hyperalgesia gender-specifically through 16 S ribosomal RNA (16 S rRNA) gene sequencing and untargeted metabolomic analysis using liquid chromatography-mass spectrometry (LC-MS). The study found that both male and female mice experienced hyperalgesia after CRS and antibiotic treatment. 16 S rRNA gene sequencing reveals gender differences in the fecal microbiota induced by CRS. The pain threshold decreased after transplanting the fecal microbiota from the male and female CRS group to the corresponding pseudo-germ-free mice. In addition, this study detected gender differences in the host gut microbiota and serum metabolism induced by fecal microbiota transplantation (FMT). Specifically, the different serum metabolites between the pseudo-germ-free mice receiving FMT from the CRS group and those from the control group were mainly involved in bile secretion and steroid hormone biosynthesis for male mice, and in taurine and hypotaurine metabolism and tryptophan metabolism for female mice. In summary, the gut microbiota participates in stress-induced hyperalgesia (SIH) with gender differences by influencing the host's gut microbiota composition and serum metabolism. Therefore, our findings provided insights into developing novel gut microbiota-associated drugs for the management of gender-specific SIH. Graphical Abstract: ga1 Highlights: Chronic restraint stress leads to different changes in the composition of fecal microbiota in male and female individuals. The fecal microbiota of stress-induced hyperalgesia mice could aggravate hyperalgesia in both male and female pseudo-germ-free mice. The fecal microbiota of stress-induced hyperalgesia mice could lead to sex-specific changes of fecal microbiota in pseudo-germ-free mice. The fecal microbiota of stress-induced hyperalgesia mice could induce sex-specific changes of serum metabolites in pseudo-germ-free mice.