Anti-inflammatory, antioxidant, antihypertensive, and antiarrhythmic effect of indole-3-carbinol, a phytochemical derived from cruciferous vegetables. Issue 2 (February 2022)

• Record Type: Journal Article
• Authors: Prado, Natalia Jorgelina; Ramirez, Daniela; Mazzei, Luciana; Parra, Micaela; Casarotto, Mariana; Calvo, Juan Pablo; Cuello carrión, Darío; Ponce Zumino, Amira Zulma; Diez, Emiliano Raúl; Camargo, Alejandra; Manucha, Walter
• Is Part Of: Heliyon. Volume 8:Issue 2(2022)
• Publication Date: 2022-02
• Subjects: Indole-3-carbinol -- Inflammation -- Hypertension -- Oxidative stress -- Arrhythmias -- Hsp70; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2022.e08989
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Background: Cardiovascular inflammation and oxidative stress are determining factors in high blood pressure and arrhythmias. Indole-3-carbinol is a cruciferous-derived phytochemical with potential anti-inflammatory and antioxidant effects. However, its implications on the modulation of cardiovascular inflammatory-oxidative markers are unknown. Objectives: To establish the effects of indole-3-carbinol on the oxidative-inflammatory-proarrhythmic conditions associated with hypertension. Materials: Histological, biochemical, molecular, and functional aspects were evaluated in 1) Culture of mouse BV-2 glial cells subjected to oxidative-inflammatory damage by lipopolysaccharides (100 ng/mL) in the presence or absence of 40 μM indole-3-carbinol (n = 5); 2) Male spontaneously hypertensive rats (SHR) and Wistar Kyoto rats receiving indole-3-carbinol (2000 ppm/day, orally) during the first 8 weeks of life (n = 15); 3) Isolated rat hearts were submitted to 10 min regional ischemia and 10 min reperfusion. Results: 1) lipopolysaccharides induced oxidative stress and increased inflammatory markers; indole-3-carbinol reversed both conditions (interleukin 6, tumor necrosis factor α, the activity of nicotinamide adenine dinucleotide phosphate oxidase, nitric oxide, inducible nitric oxide synthase, heat shock protein 70, all p < 0.01 vs lipopolysaccharides). 2) SHR rats showed histological, structural, and functional changes with increasing systolic blood pressure (154 ± 8 mmHg vs. 122 ± 7 mmHg in Wistar Kyoto rats, p < 0.01); Inflammatory-oxidative markers also increased, and nitric oxide and heat shock protein 70 decreased. Conversely, indole-3-carbinol reduced oxidative-inflammatory markers and systolic blood pressure (133 ± 8 mmHg, p < 0.01 vs. SHR). 3) indole-3-carbinol reduced reperfusion arrhythmias from 8/10 in SHR to 0/10 (p = 0.0007 by Fisher's exact test). Conclusions: Indole-3-carbinol reduces the inflammatory-oxidative-proarrhythmic process of hypertension. The nitric oxide and heat shock protein 70 are relevant mechanisms of indole-3-carbinol protective actions. Further studies with this pleiotropic phytochemical as a promising cardioprotective are guaranteed. Graphical abstract: Image 1 Highlights: Indole-3-carbinol, a cruciferous-derived compound, has cardioprotective potential. We confirmed its anti-inflammatory and antioxidant effects in vitro and in vivo. Oral administration reduced blood pressure and cardiac remodeling. In isolated hearts from hypertensive rats prevented ischemia-reperfusion arrhythmias. Heat shock protein 70 and NO contribute to indole-3-carbinol protective actions. Abstract : Indole-3-carbinol, inflammation, hypertension, oxidative stress, arrhythmias, Hsp70.