T cell‐derived exosomes containing cytokines induced keratinocytes apoptosis in oral lichen planus. (16th February 2021)

• Record Type: Journal Article
• Authors: Yang, Jing‐Ya; Tan, Ya‐Qin; Zhou, Gang
• Is Part Of: Oral diseases. Volume 28:Number 3(2022)
• Page Start: 682
• Page End: 690
• Publication Date: 2021-02-16
• Subjects: apoptosis -- cytokine -- keratinocyte -- oral lichen planus -- proliferation -- T cell‐derived exosome; Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.13795
• Languages: English
• ISSNs: 1354-523X

Abstract

Abstract: Objective: Oral lichen planus (OLP) is a T cell‐mediated inflammatory disease with uncertain etiology. Exosomes are cell‐derived vesicles containing biological cargo, being associated with the development of multiple inflammatory diseases. The present study aims to investigate the role of T cell‐derived exosomes in the pathogenesis of OLP. Methods: Exosomal marker CD63 was detected in OLP lesions by immunohistochemistry. Twenty‐three cytokines in T cell‐derived exosomes were assessed using luminex xMAP‐based assay. After co‐incubating with exosomes, the apoptosis of keratinocytes and the proliferation of Jurkat cells were assessed via flow cytometry and cell counting kit‐8 assay, respectively. Results: CD63 was highly expressed in the lymphocyte infiltrated areas of OLP lesions. OLP T cell‐derived exosomes contained upregulated interleukin‐7, ‐10, ‐12, ‐17 and downregulated interleukin‐1β, ‐5, and interferon‐γ. Both exosomes from OLP patients and controls induced the apoptosis of keratinocytes and altered their morphology. Moreover, healthy control–derived exosomes markedly inhibited the proliferation of Jurkat cells, whereas OLP‐derived exosomes exhibited no inhibitory effect. Conclusions: OLP T cell‐derived exosomes have an aberrant cytokine profile and could trigger the apoptosis of keratinocytes in vitro, which may be involved in the pathogenesis of OLP.