Implementation of wedged‐serial protein crystallography at PROXIMA‐1. (17th January 2022)

• Record Type: Journal Article
• Authors: Chaussavoine, Igor; Isabet, Tatiana; Lener, Robin; Montaville, Pierre; Vasireddi, Ramakrishna; Chavas, Leonard M. G.
• Is Part Of: Journal of synchrotron radiation. Volume 29:Part 2(2022)
• Page Start: 439
• Page End: 446
• Publication Date: 2022-01-17
• Subjects: serial crystallography -- microfluidic -- PROXIMA‐1; Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S1600577521013242
• Languages: English
• ISSNs: 0909-0495

Abstract

Abstract : The implementation of microfluidic‐based wedged‐serial protein crystallography at PROXIMA‐1 opens new possibilities for in situ data collection experiments with low sample consumption. Abstract : An approach for serial crystallography experiments based on wedged‐data collection is described. This is an alternative method for recording in situ X‐ray diffraction data on crystalline samples efficiently loaded in an X‐ray compatible microfluidic chip. Proper handling of the microfluidic chip places crystalline samples at geometrically known positions with respect to the focused X‐ray interaction area for serial data collection of small wedges. The integration of this strategy takes advantage of the greatly modular sample environment available on the endstation, which allows access to both in situ and more classical cryo‐crystallography with minimum time loss. The method represents another optional data collection approach that adds up to the already large set of methods made available to users. Coupled with the advances in processing serial crystallography data, the wedged‐data collection strategy proves highly efficient in minimizing the amount of required sample crystals for recording a complete dataset. From the advances in microfluidic technology presented here, high‐throughput room‐temperature crystallography experiments may become routine and should be easily extended to industrial use.