Elastase inhibitory activity of secondary metabolites from the fungus Virgaria nigra CF-231658. Issue 6 (19th March 2022)

• Record Type: Journal Article
• Authors: Samy, Mamdouh Nabil; Le Goff, Géraldine; Lopes, Philippe; Georgousaki, Katerina; Gumeni, Sentiljana; Almeida, Celso; Gonzalez-Menendez, Victor; Genilloud, Olga; Trougakos, Ioannis P.; Fokialakis, Nikolas; Ouazzani, Jamal
• Is Part Of: Natural product research. Volume 36:Issue 6(2022)
• Page Start: 1668
• Page End: 1671
• Publication Date: 2022-03-19
• Subjects: Virgaria nigra CF-231658 -- 2, 7-dihydroxy naphthalene -- virgaricin B -- virgaricin -- anti-elastase activity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2021.1899175
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Three known compounds were isolated from Virgaria nigra CF-231658; 2, 7-dihydroxy naphthalene (1 ), virgaricin B (2 ) and virgaricin (3 ). The isolated compounds was obtained from liquid-state and agar-supported fermentation using Amberlite XAD-16 solid-phase extraction during the cultivation step. Their structures were elucidated on the basis of 1D and 2D NMR as well as HRMS spectroscopic analyses. The isolated compounds were examined for their ability to inhibit elastase using normal human diploid fibroblasts. Compound 2 displayed the most potent activity with 76.7 ± 2.12% inhibition of the enzyme activity at 5 μM concentration. GRAPHICAL ABSTRACT: