3.5-scFv-β-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. Issue 5 (4th March 2022)

• Record Type: Journal Article
• Authors: Simonovic, Mladen; Mix, Thorsten; Glumac, Miodrag; Pejin, Boris
• Is Part Of: Natural product research. Volume 36:Issue 5(2022)
• Page Start: 1317
• Page End: 1320
• Publication Date: 2022-03-04
• Subjects: scFv-antibody -- enzyme -- fusion protein (scFv-antibody + enzyme of choice) -- TNP -- new immunoreagent tool; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1860977
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: A new protein detector of 2, 4, 6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-β-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 ± 2 fmol or 175 ± 6 pg/mL).