A magneto-controlled biocatalytic cascade with a fluorescent output. Issue 9 (14th February 2022)

• Record Type: Journal Article
• Authors: Othman, Ali; Smutok, Oleh; Kim, Yongwook; Minko, Sergiy; Melman, Artem; Katz, Evgeny
• Is Part Of: Organic & biomolecular chemistry. Volume 20:Issue 9(2022)
• Page Start: 1869
• Page End: 1873
• Publication Date: 2022-02-14
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d1ob02313f
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Magnetic nanoparticles functionalized with two different enzymes, pyruvate kinase and luciferase, were reversibly aggregated and dispersed by applying an external magnetic field, resulting in the switchable activity of the biocatalytic cascade. Abstract : A biocatalytic cascade based on concerted operation of pyruvate kinase and luciferase with a bioluminescent output was switched reversibly between low and high activity by applying an external magnetic field at different positions or removing it. The enzymes participating in the reaction cascade were bound to magnetic nanoparticles to allow their translocation or aggregation/dispersion to be controlled by the magnetic field. The reaction intensity, measured as the bioluminescent output, was dependent on the effective distances between the enzymes transported on the magnetic nanoparticles controlled by the magnets.