The protective effect of Capparis spinosa fruit on triptolide-induced acute liver injury: A metabolomics-based systematic study. (March 2022)

• Record Type: Journal Article
• Authors: Yang, Tao; Wang, Yu-Lin; Zhang, Ya-Lei; Liu, Yu-Ting; Tao, Yan-Yan; Zhou, Hua; Liu, Cheng-Hai
• Is Part Of: Journal of functional foods. Volume 90(2022)
• Publication Date: 2022-03
• Subjects: Hepatotoxic -- Traditional Chinese medicine -- Metabolism -- Liver -- Metabolomics; CSE C. spinosa fruit extract -- TP triptolide -- ALT alanine aminotransferase -- AST aspartate aminotransferase -- AKP alkaline phosphatase -- DMEM Dulbecco's Modified Eagle Medium -- MAG monoammonium glycyrrhizinate -- CGL Compound Glycyrrhizin Tablets -- SOD superoxide dismutase -- MDA malondialdehyde -- H&E hematoxylin and eosin -- TUNEL TdT-mediated dUTP nick end labeling -- SD standard deviation -- ANOVA analysis of variance -- MTP triptolide model group -- DILI drug-induced liver injury -- 5-MIAA 5-methoxyindoleacetic acid -- MT melatonin -- 5-HT 5-hydroxytryptamine -- CHB chronic hepatitis B -- HBC hepatitis B cirrhosis -- NAFLD nonalcoholic fatty liver disease; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.104989
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: The hepatoprotective effects of C. spinosa fruit extract (CSE) on triptolide (TP)-induced hepatotoxicity was found. 19 metabolites identified were associated with TP treatment and 13 metabolites were altered relative to CSE intervention. CSE-H inhibited the activities of arachidonate 5-lipoxygenase-activating protein and choline kinase alpha in the liver. Abstract: The study aimed to investigate the hepatoprotective effects of Capparis spinosa fruit extract (CSE) on triptolide (TP)-induced hepatotoxicity in vitro and in vivo. In vitro, we used a TP-induced AML-12 cell injury model and flow cytometry to determine the cell survival rate after intervention with CSE. In vivo, acute liver injury was induced by intragastric administration of TP (1000 μg/kg) to C57BL/6 mice. Two experimental groups received CSE treatment at 0.9 g/kg (CSE-Low (L)) or 2.8 g/kg (CSE-high (H)). CSE-H can significantly decrease liver cell apoptosis and ameliorated TP-induced liver injury. Furthermore, 19 metabolites identified in serum were associated with TP treatment and the levels of 13 metabolites were altered relative to TP treatment after CSE-H intervention. TP increased the activities of arachidonate 5-lipoxygenase-activating protein and choline kinase alpha in the liver, while CSE-H inhibited their activity. In conclusion, the CSE has good hepatoprotective effects on TP-induced hepatotoxicity.