Concentrations of nucleophilic sulfur species in small Indian mongoose (Herpestes auropunctatus) in Okinawa, Japan. (May 2022)

• Record Type: Journal Article
• Authors: Horai, Sawako; Abiko, Yumi; Unoki, Takamitsu; Shinkai, Yasuhiro; Akiyama, Masahiro; Nakata, Katsushi; Kunisue, Tatsuya; Kumagai, Yoshito
• Is Part Of: Chemosphere. Volume 295(2022)
• Publication Date: 2022-05
• Subjects: Mercury -- Methylmercury -- Mongoose -- Reactive sulfur species -- Sulfur -- Terrestrial mammal; CBS cystathionine β-synthase -- CSE cystathionine γ-lyase -- RSS reactive sulfur species; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.133833
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Reactive sulfur species (RSS), such as hydrogen per (poly)sulfide, cysteine per (poly)sulfide, glutathione per (poly)sulfide, and protein-bound per (poly)sulfides, can easily react with environmental electrophiles such as methylmercury (MeHg), because of their high nucleophilicity. These RSS are produced by enzymes such as cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) and are found in mammalian organs. Organs of wildlife have not been analyzed for hydrogen sulfide, cysteine, glutathione, and RSS. In this study, low molecular weight nucleophilic sulfur substances, including RSS, were quantified by stable isotope dilution assay-based liquid chromatography-mass spectrometry using β-(4-hydroxyphenyl)ethyl iodoacetamide to capture the target chemicals in the small Indian mongoose which species possesses high mercury content as same as some marine mammals. Western blotting revealed that the mongoose organs (liver, kidney, cerebrum, and cerebellum) contained proteins that cross-reacted with anti-CBS and CSE antibodies. The expression patterns of these enzymes were similar to those in mice, indicating that mongoose organs contain CBS and CSE. Moreover, bis-methylmercury sulfide (MeHg)2 S, which is a low toxic compound in comparison to MeHg, was found in the liver of this species. These results suggest that the small Indian mongoose produces RSS and monothiols associated with detoxification of electrophilic organomercury. The animals which have high mercury content in their bodies may have function of mercury detoxification involved not only Se but also RSS interactions. Graphical abstract: Image 1 Highlights: Mongoose organs contain CBS and CSE, which generate reactive sulfur species. Cysteine, glutathione, H2 S, and their persulfides are present in mongoose organs. Significant correlations exist between sulfur compounds and Hg in some organs. (MeHg)2 S was found in the mongoose liver.