Immunomodulatory effects of Companilactobacillus allii WiKim39 and Lactococcus lactis WiKim0124 isolated from kimchi on lipopolysaccharide-induced RAW264.7 cells and dextran sulfate sodium-induced colitis in mice. (March 2022)

• Record Type: Journal Article
• Authors: Yun, Ye-Rang; Lee, Moeun; Song, Jung Hee; Choi, Eun Ji; Chang, Ji Yoon
• Is Part Of: Journal of functional foods. Volume 90(2022)
• Publication Date: 2022-03
• Subjects: Kimchi -- Companilactobacillus allii WiKim39 -- Lactococcus lactis WiKim0124 -- Vegetable juice fermentation -- Anti-inflammation; PPAR-γ peroxisome proliferator-activated receptor γ (PPAR-γ) -- LAB lactic acid bacteria -- TRP transient receptor potential -- Nrf2 nuclear factor (erythroid-derived 2)-like-2 factor -- IL-10 interleukin-10 -- DSS dextran sulfate sodium -- VJ vegetable juice -- LPS lipopolysaccharide -- PHWE pressurized hot water extraction -- OD optical density -- NO nitric oxide -- NOR normal group -- AST aspartate transaminase -- ALT alanine transaminase -- H&E hematoxylin and eosin -- IL-1β interleukin 1 beta -- IL-6 interleukin 6 -- TNFα tumor necrosis factor-alpha -- MCP-1 monocyte chemoattractant protein 1 -- iNOS inducible nitric oxide synthase -- COX-2 cyclooxygenase 2 -- NF-κB nuclear factor kappa B; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.104969
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Vegetable juice was fermented with Companilactobacillus allii WiKim39 or Lactococcus lactis Wikim0124 isolated from kimchi. VJ + WiKim39 and VJ + WiKim0124 exhibited anti-inflammatory effects in LPS-induced RAW264.7 cells. VJ + WiKim39 and VJ + WiKim0124 exhibited anti-inflammatory effects in DSS-induced colitis in mice. The anti-inflammatory effects of VJ + WiKim39 and VJ + WiKim0124 were established via the NF-kB pathway in vitro and in vivo . Abstract: Lactic acid bacteria (LAB) isolated from kimchi has beneficial antioxidant, anti-obesity, anti-cancer and immunomodulatory effects. Here, we investigated the immunomodulatory effects of vegetable juice (VJ) fermented with Companilactobacillus allii WiKim39 (VJ + WiKim39) or Lactococcus lactis WiKim0124 (VJ + WiKim0124) isolated from kimchi, in vitro and in vivo . Nitric oxide (NO) production and levels of inflammatory markers were significantly lower in lipopolysaccharide (LPS)-stimulated RAW264.7 cells treated with heat-inactivated VJ + WiKim39 and heat-inactivated VJ + WiKim0124 than in untreated cells ( P < 0.05). In mice with dextran sulfate sodium (DSS)-induced colitis, oral administration of VJ + WiKim39 and VJ + WiKim0124 attenuated DSS-induced decrease in colon length and mucosal epithelia. Moreover, VJ + WiKim39 and VJ + WiKim0124 significantly downregulated several inflammatory markers ( P < 0.05). Overall, WiKim39 and WiKim0124 showed immunomodulatory activity in vitro and in vivo and thus, they could be used in health promoting fermented foods.