Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents. Issue 4 (April 2022)

• Record Type: Journal Article
• Authors: Fujioka-Kobayashi, M.; Marjanowski, S.D.; Kono, M.; Hino, S.; Saulacic, N.; Schaller, B.
• Is Part Of: International journal of oral & maxillofacial surgery. Volume 51:Issue 4(2022)
• Page Start: 566
• Page End: 575
• Publication Date: 2022-04
• Subjects: bone morphogenetic proteins -- BMP-9 -- denosumab -- antiresorptive agents -- bone regeneration; Mouth -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Dentistry -- Periodicals; Dentistry, Operative; Oral Surgical Procedures; Surgery, Oral; Dentistry; Maxilla -- Surgery; Mouth -- Surgery; Electronic journals; Periodicals; 617.52059
• DOI: 10.1016/j.ijom.2021.08.014
• Languages: English
• ISSNs: 0901-5027

Abstract

Abstract: The aim of the present study was to investigate the effects of recombinant human (rh)BMP-9 on bone regenerative potential in a mouse model of antibody-mediated antiresorptive therapy (AMART). A monoclonal anti-murine receptor activator of nuclear factor-kappa B ligand (RANKL) antibody (mAb) was used to create an AMART model in mice. rhBMP-9 combined with collagen membrane was implanted in calvarial defects in mAb-treated mice. After 4 weeks, the bone formative potential in the defects was evaluated by micro-computed tomography and histological approaches. The groups implanted with rhBMP-9-containing collagen membranes demonstrated substantial osteopromotive potential, with significantly greater new bone volume (Sham + BMP-9 group; 0.86 ± 0.29 mm 3 and mAb + BMP-9 group; 0.64 ± 0.16 mm 3 ) than control PBS-membranes (Sham + PBS group; 0.44 ± 0.29 mm 3 and mAb + PBS group; 0.24 ± 0.12 mm 3 ) in both sham and mAb-treated mice. In line with in vivo study, bone marrow cells isolated from both sham and mAb-treated mice confirmed greater osteogenic potential upon stimulation with rhBMP-9 in vitro. These findings suggest for the first time that local rhBMP-9 administration might be a strategy to accelerate bone regeneration in the context of AMART.