Engineered Halomonas spp. for production of l-Lysine and cadaverine. (April 2022)

• Record Type: Journal Article
• Authors: Zhao, Cuihuan; Zheng, Taoran; Feng, Yinghao; Wang, Xuan; Zhang, Lizhan; Hu, Qitiao; Chen, Jinchun; Wu, Fuqing; Chen, Guo-Qiang
• Is Part Of: Bioresource technology. Volume 349(2022)
• Publication Date: 2022-04
• Subjects: Halomonas -- L-Lysine -- Cadaverine -- PHB -- Synthetic Biology; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.126865
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Recombinant Halomonas bluephagenesis produced lysine from glucose. The cadaverine biosynthetic pathway was constructed in Halomonas campaniensis. Halomonas spp. can biosynthesize cadaverine from glucose as a sole carbon source. H. campaniensis LC-9 -ldcC-lysP efficiently produced cadaverine from lysine. Abstract: Cadaverine, a derivative of l -lysine, has been used as a monomer for the synthesis of bio-based nylon-5, 6. This study engineered Halomonas bluephagenesis TD1.0 by blocking the feedback inhibition, overexpressing the key l -lysine synthesis genes, strengthening the l -lysine export system and increasing the supply of oxaloacetate for production of l -lysine in the supernatant and PHB in the cells. Subsequently, cadaverine biosynthetic pathway was constructed in H. campaniensis LC-9 to improve the efficiency of de novo cadaverine biosynthesis which combines l -lysine producing H. bluephagenesis TDL8-68–259 and cadaverine producing H. campaniensis LC-9 -ldcC-lysP . When H. campaniensis LC-9- ldcC-lysP was used as a whole cell catalysis for cadaverine production, the conversion efficiency of l -lysine to cadaverine reached 100% in the presence of 0.05% Triton X-100 for cell membrane permeability enhancement, resulting in 118 g L -1 cadaverine formed in the fermentor. Thus, Halomonas spp. have been successfully constructed for l -lysine and cadaverine production.