Dietary supplementation with okara and Bacillus coagulans lilac-01 improves hepatic lipid accumulation induced by cholic acids in rats. (March 2022)

• Record Type: Journal Article
• Authors: Lee, Yeonmi; Tanaka, Yasutake; Iwasaki, Wakana; Yokoyama, Fumika; Joe, Ga-Hyun; Tsuji, Misaki; Nose, Takuma; Tada, Koji; Hanai, Taketo; Hori, Shota; Shimizu, Hidehisa; Minamida, Kimiko; Miwa, Kazunori; Ishizuka, Satoshi
• Is Part Of: Journal of functional foods. Volume 90(2022)
• Publication Date: 2022-03
• Subjects: Symbiotic -- Hepatic lipid accumulation -- Cholic acid -- Okara -- Rats; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.104991
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Cholic acid supplementation induced lipid accumulation in the liver and a reduction in iron concentration in rats. Dietary supplementation with okara, a by-product of tofu manufacturing from soybean, and Bacillus coagulans lilac-01, protects against leaky gut and lipid accumulation with reducing the expression of fatty acid synthase ( Fasn ) in the liver and enhancement of fecal triglyceride and non-12αhydroxylated bile acid excretion accompanied by an increase in cecal propionic acid concentration. Highlights: Okara and Bacillus coagulans lilac-01 is a dietary synbiotic (Syn) Cholic acid (CA) induces lipid accumulation in rats in a 2-week study. Syn ameliorates CA-induced hepatic lipid accumulation and leaky gut. Syn enhances fecal excretion of triglyceride and non-12αhydroxylated bile acids. Abstract: A dietary symbiotic supplement of okara, a by-product of tofu manufacturing with Bacillus coagulans lilac-01 found to reduce secondary bile acid (BA) in a rat study and cholic acid (CA), a primary 12α-hydroxylated (12αOH) BA, induces lipid accumulation in rats. In this study, we investigated whether dietary supplementation of a synbiotic with okara and Bacillus coagulans lilac-01 improves CA-induced hepatic lipid accumulation. Male Wistar/ST rats (4 weeks old) were fed with or without CA-supplementation combined with the synbiotic for 2 weeks. The CA diet increased hepatic triglycerides and the synbiotic diet in combination with CA normalized liver triglyceride concentration accompanied by suppression of hepatic fatty acid synthase expression, enhancement of fecal triglyceride excretion, and increase in fecal non-12αOH BA excretion. Dietary supplementation with the okara synbiotic ameliorated hepatic lipid accumulation probably by reducing de novo lipogenesis and enhancing fecal triglyceride excretion.