Cover Feature: A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress (Anal. Sens. 1/2022). Issue 1 (3rd December 2021)

• Record Type: Journal Article
• Authors: Shao, Shuai; Deng, Sha; Jiang, Qingyun; Zhang, Hangyu; Zhang, Zhengyao; Li, Na; Cong, Fengyu; Tiihonen, Timo; Liu, Bo
• Is Part Of: Analysis & Sensing. Volume 2:Issue 1(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-03
• Subjects: biosensors -- focal adhesions -- FRET -- paxillin -- shear stress; Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; 543
• DOI: 10.1002/anse.202100061
• Languages: English
• ISSNs: 2629-2742

Abstract

Abstract : The cover shows a DNA‐encoded biosensor based on a Fluorescence Resonance Energy Transfer (FRET) technique named PaxTS. The biosensor consists of an LD12 domain, an LIM1234 domain, a nano‐spring and a pair of fluorescent proteins to convert the tension across paxillin to FRET efficiency in living cells, indicating that there exists a force transmission pathway described as 'membrane‐cytoskeleton‐FAs' and involves paxillin upon mechanical force. More information can be found in the Full Paper by Bo Liu and co‐workers .