Inactivation of antithrombin III in synovial fluid from patients with rheumatoid arthritis. Issue 3 (1st March 1998)

• Record Type: Journal Article
• Authors: Jones, H W; Bailey, R; Zhang, Z; Dunne, K A; Blake, D R; Cox, N L; Morris, C J; Winyard, P G
• Is Part Of: Annals of the rheumatic diseases. Volume 57:Issue 3(1998)
• Page Start: 162
• Page End: 165
• Publication Date: 1998-03-01
• Subjects: antithrombin III -- thrombin -- rheumatoid arthritis -- synovial fluid; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/ard.57.3.162
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : OBJECTIVE: To investigate the thrombin inhibitory capacity of antithrombin III in the inflamed human joint. METHODS: Thrombin inhibitory capacity was measured, using a kinetic spectophotometric method, in matched plasma and synovial fluid samples of patients with rheumatoid arthritis (n=22) and osteoarthritis (n=16), together with normal control plasma samples (n=13). In the same samples, the concentration of antithrombin III was also determined by the method of radial immunodiffusion. The combination of these measurements allowed the calculation of the specific thrombin inhibitory capacity of these samples. RESULTS: An increased concentration of antithrombin III in rheumatoid compared with osteoarthritic synovial fluid was noted (p<0.05). However, there was a significant depression in the specific activity of antithrombin III in rheumatoid synovial fluid when compared with matched plasma samples (p<0.001) or with osteoarthritic synovial fluid (p<0.05). CONCLUSION: In rheumatoid synovial fluid the thrombin inhibitory capacity of antithrombin III is disproportionately depressed relative to the concentration of antithrombin III, indicating the inactivation of antithrombin III in the rheumatoid joint.