Thermo-responsive microcarriers based on poly(N-isopropylacrylamide). (June 2015)

• Record Type: Journal Article
• Authors: Zhang, Jinnan; Cui, Zhanfeng; Field, Robert; Moloney, Mark G.; Rimmer, Stephen; Ye, Hua
• Is Part Of: European polymer journal. Volume 67(2015:Jun.)
• Page Start: 346
• Page End: 364
• Publication Date: 2015-06
• Subjects: AAc acrylic acid -- ACVA 4, 4′-azobis(4-cyanovaleric acid) -- AEMA N-aminoethyl methacrylate -- AMPTMA (3-acrylamidopropyl)-trimethylammonium chloride -- APS ammonium persulfate -- ATRP atom transfer radical polymerization -- BPOEA 2-(2-bromopropionyloxy) ethyl acrylate -- bpy 2, 2′-bipyridine -- CHO Chinese hamster ovary -- CME chloromethyl methyl ether -- CRP controlled/living radical polymerization -- DCC N, N′-dicyclohexylcarbodiimide -- DVB divinylbenzene -- ECM extracellular matrix -- ECP ethyl-2-chloropropionate -- EDC N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride -- EDTA ethylenediaminetetraacetic acid -- EEDQ 1-(ethoxycarbonyl)-2-ethoxy-1, 2-dihydroquinoline -- FCs fetal chondrocytes -- GRGDY Glycine-Arginine-Glycine-Aspartic acid-Tyrosine -- hBMMSCs human bone marrow-derived mesenchymal stem cells -- HBPS hyperbranched poly(siloxysilane) -- HEA hydroxyethyl acrylate -- HMTETA 1, 1, 4, 7, 10, 10-hexamethyltriethylenetetramine -- KPS potassium persulfate -- LCST lower critical solution temperature -- MBAAm N, N′-methylenebis(acrylamide) -- Me4Cycloam 1, 4, 8, 11-tetramethyl-1, 4, 8, 11-tetraazacyclotetradecane -- Me6TREN tris[2-(dimethylamino)ethyl]amine -- MPA 3-mercaptopropionic acid -- NHS N-hydroxysuccinimide -- NIPAAm N-isopropylacrylamide -- NMP nitroxide-mediated polymerization -- PEG poly(ethylene glycol) -- PGMA poly(glycidyl methacrylate) -- PHEMA poly(2-hydroxyethylmethacrylate) -- PIMP photoiniferter-mediated polymerization -- PMDETA N, N, N′, N″, N″-pentamethyldiethylenetriamine -- PNIPAAm poly(N-isopropylacrylamide) -- PNIPAAm-COOH carboxyl-terminated PNIPAAm -- PNIPAAm-NH2 amine-terminated PNIPAAm -- poly(NIPAAm-co-AAc) poly(N-isopropylacrylamide-co-acrylic acid) -- PS polystyrene -- PVA polyvinyl acetate -- RAFT reversible addition-fragment chain transfer -- RGD Arginine-Glycine-Aspartic acid -- SEC size-exclusion chromatography -- Sn(EH)2 tin (II) 2-ethylhexanoate -- TMED N, N, N′, N′-tetramethylethylenediamine -- UV ultraviolet -- VA-086 2, 2′-azobis[2-methyl-N-(2-hydroxyethyl)propionamide] -- W/O water-in-oil; Thermo-responsive microcarrier -- Poly(N-isopropylacrylamide) -- Cell culture -- Polymerization; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2015.04.013
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Highlights: Microcarrier cell culture systems are useful for cell culture amplification. Surface PNIPAAm releases cells by temperature adjustment. This review presents methods to prepare PNIPAAm microcarriers. The efficiency of methods is compared. Abstract: Microcarrier cell culture systems provide an attractive alternative to the conventional monolayer cell culture for cell amplification, due to their high surface area-to-volume ratio. Unlike enzymatic methods for removing cells from microcarriers after cell culture, which can lead to irreversible damage of the cells, microcarriers which release cells by temperature adjustment have been developed. This was achieved by grafting a temperature-responsive polymer, poly( N -isopropylacrylamide) (PNIPAAm), on the microcarrier surface. This review comprehensively presents various methods to prepare such thermo-responsive microcarriers based on PNIPAAm. These methods include the grafting-to technique, grafting-from technique, grafting-through technique, along with methods leading to PNIPAAm hydrogel beads, seeded polymerization, and non-covalent adsorption. The methods for controlling PNIPAAm grafting density, molecular weight and molecular architecture are also outlined. Further, the efficiency of cell attachment, proliferation and thermally-induced detachment of such thermo-responsive microcarriers is introduced and compared.