Serum samples of patients with rheumatoid arthritis contain a specific autoantibody to "denatured" aldolase A in the osteoblast-like cell line, MG-63. Issue 3 (1st March 1999)

• Record Type: Journal Article
• Authors: Ukaji, Fumio; Kitajima, Isao; Kubo, Toshikazu; Shimizu, Choji; Nakajima, Toshihiro; Maruyama, Ikuro
• Is Part Of: Annals of the rheumatic diseases. Volume 58:Issue 3(1999)
• Page Start: 169
• Page End: 174
• Publication Date: 1999-03-01
• Subjects: autoantibody -- aldolase A -- denature -- rheumatoid arthritis; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/ard.58.3.169
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : OBJECTIVE: To identify rheumatoid arthritis (RA) specific autoantibody and its antigen in the human osteoblast-like cell line, MG-63. METHODS: MG-63 cell extract was subjected to western blotting by using RA and normal serum samples as probes. The autoantigen was purified and its N-terminal sequence was determined by automated Edman degradation. The reactivity of denatured aldolase A was evaluated by immunoblotting. Screening by enzyme linked immunosorbent assay (ELISA) using the autoantibody was performed. RESULTS: 40 kDa protein was found only in the RA serum samples and it was identified as aldolase A. A polyclonal antibody for rabbit muscle aldolase A bound to the 40 kDa protein and reacted in preference with the denatured enzyme. Using ELISA for denatured rabbit aldolase A, the autoantibody was found in approximately 10% of RA patients, whereas it was not found in the other arthropathy and healthy adults. CONCLUSION: This 40 kDa anti-aldolase A autoantibody, which was identified only in serum samples of RA patients with severe bone erosion, could be related to a certain event that induces RA specific joint destructions.