Dynamic biological interfaces functionalized fructose-responsive immunomagnetic beads for high-efficient and high-purity exosome enrichment. (January 2022)

• Record Type: Journal Article
• Authors: Li, Guohao; Zhu, Nanhang; Cheng, Jia; Zhang, Yujia; Yu, Yue; Zhang, Xiaolin; Yi, Qiangying; Wu, Yao
• Is Part Of: Materials & design. Volume 213(2022)
• Publication Date: 2022-01
• Subjects: Exosomes -- Immunomagnetic beads -- Fructose-responsive -- Capture and release; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2021.110366
• Languages: English
• ISSNs: 0264-1275

Abstract

Graphical abstract: Dynamic biological interfaces of the fructose-responsive immunomagnetic beads are capable to realize a fructose-triggered molecule change and the captured exosomes with high purity can be released biofriendly and efficiently. Highlights: Fructose-responsive dynamic biological interfaces were constructed to functionalize the immunomagnetic beads. Fabricated immunomagnetic beads enabled high-efficient exosome capture and release under mild conditions. Released exosomes remained good bioactivity, higher purity and better preservation of tumorous molecular information. Abstract: Enrichment of exosomes with high structural and functional integrity is a critically important step before downstream applications in biological and clinical domains. In this work, dynamic biological interfaces functionalized fructose-responsive immunomagnetic beads (Fr-IMBs) were fabricated by incorporating the dynamic biological interfaces composed of dopamine and 4-carboxyphenylboronic acid with immunomagnetic beads. These fabricated Fr-IMBs were successfully applied to capture the MCF-7 exosomes with a high capture efficacy of 91.0%. After incubation with fructose, the molecular structure of the dopamine-boronic acid complexation can be rearranged and the antibody (anti-CD63) bound exosomes were released competitively, with a yield greater than 79.8%. Those exosomes that underwent both immunomagnetic separation and fructose triggered release remained their good bioactivity to promote MCF-7 cell migration. Better yet, exosomes enriched by Fr-IMBs possessed higher purity and better preservation of tumor-related molecular information of the exosomal proteins than those obtained through the ultracentrifugation approach, as proved by the comparative proteomics. These proposed sugar-responsive immunomagnetic beads may serve as biofriendly candidates in enriching useful biomarkers (e.g., cells, nucleic acids, proteins, etc.) efficiently from complex biological samples with high purity.