Supramolecular CRISPR-OFF switches with host–guest chemistry. Issue 3 (31st January 2022)

• Record Type: Journal Article
• Authors: Xiong, Wei; Liu, Xingyu; Qi, Qianqian; Ji, Huimin; Liu, Fengbo; Zhong, Cheng; Liu, Simin; Tian, Tian; Zhou, Xiang
• Is Part Of: Nucleic acids research. Volume 50:Issue 3(2022)
• Page Start: 1241
• Page End: 1255
• Publication Date: 2022-01-31
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkac008
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) technology is a powerful tool in biology and medicine. However, the safety and application of this technology is hampered by excessive activity of CRISPR machinery. It is particularly important to develop methods for switching off CRISPR activity in human cells. The current study demonstrates the concept of supramolecular CRISPR-OFF switches by employing host-guest chemistry. We demonstrate that the CRISPR systems show considerable tolerance to adamantoylation on guide RNAs (gRNAs), whereas supramolecular complexation tremendously affects the function of adamantoyl gRNAs. Host–guest chemistry is demonstrated to be novel and effective tools to reduce unwanted excessive activities of CRISPR complexes in human cells. This work indicates considerable potential of supramolecular strategy for controlling and enhancing CRISPR systems.