The Pentameric Nucleoplasmin Fold Is Present in Drosophila FKBP39 and a Large Number of Chromatin-Related Proteins. Issue 10 (22nd May 2015)

• Record Type: Journal Article
• Authors: Edlich-Muth, Christian; Artero, Jean-Baptiste; Callow, Phil; Przewloka, Marcin R.; Watson, Aleksandra A.; Zhang, Wei; Glover, David M.; Debski, Janusz; Dadlez, Michal; Round, Adam R.; Forsyth, V. Trevor; Laue, Ernest D.
• Is Part Of: Journal of molecular biology. Volume 427:Issue 10(2015:May 15)
• Page Start: 1949
• Page End: 1963
• Publication Date: 2015-05-22
• Subjects: SANS small-angle neutron scattering -- SAXS small-angle X-ray scattering -- NOE nuclear Overhauser enhancement -- HSQC heteronuclear single quantum coherence -- PPI peptidyl proline isomerase; histone chaperone -- nucleoplasmin -- FKBP -- structure determination -- NMR; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2015.03.010
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: Nucleoplasmin is a histone chaperone that consists of a pentameric N-terminal domain and an unstructured C-terminal tail. The pentameric core domain, a doughnut-like structure with a central pore, is only found in the nucleoplasmin family. Here, we report the first structure of a nucleoplasmin-like domain (NPL) from the unrelated Drosophila protein, FKBP39, and we present evidence that this protein associates with chromatin. Furthermore, we show that two other chromatin proteins, Arabidopsis thaliana histone deacetylase type 2 (HD2) and Saccharomyces cerevisiae Fpr4, share the NPL fold and form pentamers, or a dimer of pentamers in the case of HD2. Thus, we propose a new family of proteins that share the pentameric nucleoplasmin-like NPL domain and are found in protists, fungi, plants and animals. Graphical abstract: Highlights: The FKBP39 N-terminal domain has the nucleoplasmin fold and forms a homo-pentamer. FKBP39 binds to chromatin, interacts with histones and binds divalent metal ions. Other proteins such as the yeast Fpr4 and plant HD-tuins are expected to have the same N-terminal domain with similar properties.