MiR-124 promotes bone marrow mesenchymal stem cells differentiation into neurogenic cells for accelerating recovery in the spinal cord injury. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Zhao, Yong; Jiang, Hui; Liu, Xin-Wei; Xiang, Liang-Bi; Zhou, Da-Peng; Chen, Jian-Ting
• Is Part Of: Tissue & cell. Volume 47:Issue 2(2015)
• Page Start: 140
• Page End: 146
• Publication Date: 2015-04
• Subjects: microRNA -- Bone marrow -- MSCs -- Neurogenic cells; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2015.01.007
• Languages: English
• ISSNs: 0040-8166

Abstract

Highlights: The miR-124 induced formation of neurogenic cells from bone marrow mesenchymal stem cells via down-regulation of PTBP1 and activation of PTBP2. The neurogenic cells derived from bone marrow mesenchymal stem cells can take part in recovery of spinal cord injury. Abstract: In this research, mouse BMMSCs were isolated from bone marrow, induced to differentiate into neurogenic cells in vitro, and transplanted into the injured spinal cord after over-expression of miR-124. The results showed that the BMMSCs could induce the differentiation to neurogenic cells under the special condition medium, but when the miR-124 was over-expressed, the differentiation efficiency of neurogenic cells from BMMSCs could be promoted. This reason was demonstrated that polypyrimidine tract-binding protein 1 (PTBP1) showed a repressor for polypyrimidine tract-binding protein 2 (PTBP2) during neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. miR-124 promoted neurogenic cells from BMMSCs were successful colonized into injured spinal cord for participation in tissue-repair. In conclusion, our research shows that the miR-124 promoted the differentiation of neuronal cells from BMMSCs, and this mechanism was miR-124 reduced the expression of PTBP1, increased the expression of PTBP2.