Effects of quercetin on human oral keratinocytes during re-epithelialization: An in vitro study. (November 2018)

• Record Type: Journal Article
• Authors: Hujiahemaiti, Miyashaer; Sun, Xiaolin; Zhou, Jing; Lv, Huixin; Li, Xue; Qi, Manlin; Chi, Minghan; Li, Chunyan; Zhou, Yanmin
• Is Part Of: Archives of oral biology. Volume 95(2018)
• Page Start: 187
• Page End: 194
• Publication Date: 2018-11
• Subjects: Quercetin -- Oral keratinocytes -- α6β4 integrin -- Re-epithelization; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2018.08.004
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: Quercetin can modulate the inflammation induced by P. gingivalis lipopolysaccharide. Quercetin enhances TGF-β3 expression without affecting TGF-β1 production. Quercetin enhances HOK proliferation by promoting TGF-β3 secretion in vitro . Abstract: Background: The width of keratinized mucosa plays an important role in esthetic and functional outcomes of dental implants. Lack of keratinized mucosa may lead to poor oral hygiene and greater soft-tissue recession. This study aimed at assessing the potential of quercetin in promoting human oral keratinocyte (HOK) proliferation and re-epithelialization in vitro . Materials and Methods: HOK were detected in the absence or presence of test substances. The Cell Counting Kit-8 was used to assess cell viability and proliferation capacity. Re-epithelization was assessed using a keratinocyte monolayer scratch assay. Cell migration was monitored via Transwell chambers. Porphyromonas gingivalis lipopolysaccharide was used to stimulate keratinocytes for mimicking the inflammatory situation. mRNA expression of inflammatory cytokines (interleukin-1beta, IL-1β and tumor necrosis factor alpha, TNF-α), cell adhesion molecules (Integrin-α6, Integrin-β4), and growth factors (transforming growth factor beta 1, TGF-β1 and transforming growth factor beta 3, TGF-β3) were estimated using RT-qPCR. Protein contents of TGF-β1 and TGF-β3 were investigated by enzyme-linked immunosorbent assay. Results: Multiplex analysis revealed that quercetin enhances HOK proliferation via an upregulation of adhesion molecules (Integrin-α6β4). Additionally, re-epithelialization rate was significantly greater in the presence of quercetin than in the control (P < 0.01). Furthermore, 20 μM of quercetin increases both mRNA and protein levels of TGF-β3 under basal and wound conditions without affecting TGF-β1 production. Expressions of pro-inflammatory cytokines were downregulated by quercetin treatment. Conclusion: Quercetin promotes HOKs proliferation and oral re-epithelialization in vitro.