Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides). Issue 2 (October 2015)

• Record Type: Journal Article
• Authors: Guo, Chuanyu; Cui, Huachun; Ni, Songwei; Yan, Yang; Qin, Qiwei
• Is Part Of: Developmental and comparative immunology. Volume 52:Issue 2(2015)
• Page Start: 226
• Page End: 235
• Publication Date: 2015-10
• Subjects: microRNAs -- Grouper -- SGIV -- Deep sequencing -- miRNA microarray -- Functional annotation; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.05.014
• Languages: English
• ISSNs: 0145-305X

Abstract

Highlights: miRNAs profile of grouper was performed by Solexa deep sequencing and microarray. A total of 107 conserved and 9 novel miRNAs were identified. Many miRNAs were differentially regulated during SGIV infection in vivo and in vitro . Target prediction indicated that a number of miRNAs involved in immunity. Abstract: microRNAs (miRNAs) are an evolutionarily conserved class of non-coding RNA molecules that participate in various biological processes. Employment of high-throughput screening strategies greatly prompts the investigation and profiling of miRNAs in diverse species. In recent years, grouper ( Epinephelus spp.) aquaculture was severely affected by iridoviral diseases. However, knowledge regarding the host immune responses to viral infection, especially the miRNA-mediated immune regulatory roles, is rather limited. In this study, by employing Solexa deep sequencing approach, we identified 116 grouper miRNAs from grouper spleen-derived cells (GS). As expected, these miRNAs shared high sequence similarity with miRNAs identified in zebrafish ( Danio rerio ), pufferfish ( Fugu rubripes ), and other higher vertebrates. In the process of Singapore grouper iridovirus (SGIV) infection, 45 and 43 miRNAs with altered expression (>1.5-fold) were identified by miRNA microarray assays in grouper spleen tissues and GS cells, respectively. Furthermore, target prediction revealed 189 putative targets of these grouper miRNAs.