Expression of tumor suppressor genes in channel catfish after bacterial infections. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Mu, Weijie; Yao, Jun; Zhang, Jiaren; Liu, Shikai; Wen, Haishen; Feng, Jianbin; Liu, Zhanjiang
• Is Part Of: Developmental and comparative immunology. Volume 48:Issue 1(2015)
• Page Start: 171
• Page End: 177
• Publication Date: 2015-01
• Subjects: Tumor suppressor gene -- Fish -- Disease infection -- P53 -- Genome -- Expression; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2014.10.004
• Languages: English
• ISSNs: 0145-305X

Abstract

Highlights: Identification of 21 tumor suppressor genes from channel catfish. Two P53 genes are found in channel catfish which were supported by phylogenetic and syntenic analyses. Distinct expression patterns were observed for TSGs after bacterial infections. Abstract: Tumor suppressor genes are negative regulators of tumor formation. While their anti-tumor functions have been well studied, they have been found to be also involved in immune responses and innate immunity. In this study, 21 tumor suppressor genes in channel catfish ( Ictalurus punctatus ) were characterized. Phylogenetic and syntenic analyses allowed annotation of all 21 catfish tumor suppressor genes. The expression profiles of the 21 catfish tumor suppressor genes were determined using the RNA-Seq datasets. After Edwardsiella ictaluri infection, expression of five of the 21 tumor suppressor genes was up-regulated at 3 days in the intestine, and four of the 21 genes were up-regulated in the liver 14 days post-infection. With Flavobacterium columnare infection, seven genes were up-regulated in the gill at 48 h post-infection. These results expanded our knowledge on the tumor suppressor genes in teleosts, setting a foundation for future studies to unravel functions of tumor suppressor genes in response to stresses, particularly after bacterial disease infections.