Lipid mediators of inflammation as novel plasma biomarkers to identify patients with bacteremia. Issue 5 (May 2015)

• Record Type: Journal Article
• Authors: To, Kelvin K.W.; Lee, Kim-Chung; Wong, Samson S.Y.; Lo, Ka-Ching; Lui, Yin-Ming; Jahan, Akhee S.; Wu, Andrea L.; Ke, Yi-Hong; Law, Chun-Yiu; Sze, Kong-Hung; Lau, Susanna K.P.; Woo, Patrick C.Y.; Lam, Ching-Wan; Yuen, Kwok-Yung
• Is Part Of: Journal of infection. Volume 70:Issue 5(2015)
• Page Start: 433
• Page End: 444
• Publication Date: 2015-05
• Subjects: Bacteremia -- Biomarker -- Metabolomic -- Lipid -- Organic acid -- Plasma; Infection -- Periodicals; Bacterial Infections -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.905
• DOI: 10.1016/j.jinf.2015.02.011
• Languages: English
• ISSNs: 0163-4453

Abstract

Summary: Objectives: Rapid diagnostic tests for bacteremia are important for early treatment to improve clinical outcome. We sought to identify plasma biomarkers that can identify patients with bacteremia using an untargeted global metabolomic analysis. Methods: Plasma metabolomic profiles were analyzed for 145 adult patients with (cases) and without (controls) bacteremia using ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). All metabolites were compared between cases and controls using a 2-tier filtering approach, and each metabolite underwent receiver operating characteristic (ROC) curve analysis. Individual metabolites that distinguish between cases and controls were characterized. Subgroup analysis was performed to identify metabolites with prognostic significance. Results: After 2-tier filtering, 128 molecular features were identified to be potential biomarkers that could distinguish cases from controls. Five metabolites had an area under the ROC curve (AUC) of >0.8 in ROC curve analysis, including a sphingolipid, an acylcarnitine, a fatty acid ester, and 2 glycerophosphocholines. These metabolites could distinguish cases from controls in the unsupervised hierarchical clustering analysis. Subgroup analysis of bacteremic patients showed that the level of trans-2, 3, 4-trimethoxycinnamate was lower in fatal than non-fatal cases. Conclusions: Plasma lipid mediators of inflammation can distinguish bacteremia cases from non-bacteremia controls. These biomarkers may be used as targets for rapid test in clinical practice. Highlights: We compared the metabolomic profile of patients with bacteremia and those without bacteremia. Two glycerophosphocholines were downregulated in bacteremia. A sphingosine, an acylcarnitine and an arachidonic acid were upregulated in bacteremia. Trans-2, 3, 4-trimethoxycinnamate was lower in fatal than non-fatal bacteremia cases.