Differential expression patterns of glycosphingolipids and C‐type lectin receptors on immune cells in absence of functional regulatory T cells. Issue 4 (1st August 2020)

• Record Type: Journal Article
• Authors: Jirmo, Adan C.; Rossdam, Charlotte; Grychtol, Ruth; Happle, Christine; Gerardy‐Schahn, Rita; Buettner, Falk F. R.; Hansen, Gesine
• Is Part Of: Immunity, inflammation and disease. Volume 8:Issue 4(2020)
• Page Start: 512
• Page End: 522
• Publication Date: 2020-08-01
• Subjects: CD4+ T cells -- dendritic cells -- FOXP3‐deficient -- glycosphingolipids; Immunology -- Periodicals; Immunity -- Periodicals; Inflammation -- Periodicals; 616.079
• DOI: 10.1002/iid3.334
• Languages: English
• ISSNs: 2050-4527

Abstract

Abstract: Background: Glycosylation is a common and complex type of protein posttranslational modification. Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site‐specific relative abundances of individual glycan structures and glycosylation patterns. However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation. Hypothesis: Glycosphingolipid patterns on immune cells of forkhead‐box‐P3‐deficient scurfy mice differs from those on wild‐type immune cells. Methods: T cells and dendritic cells were isolated from spleens of either wild‐type or age‐matched scurfy mice. Glycosphingolipids of CD4 + T cells and splenic dendritic cells from wild‐type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser‐induced fluorescence detection (xCGE‐LIF). In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C‐type lectin receptors on antigen‐presenting cells from various organs of both wild‐type and scurfy mice. Results: We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild‐type and scurfy mice. Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC‐7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen‐presenting cells. Conclusion: We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells. Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition. Abstract : (a) Attenuated levels of glycosphingolipids in splenic dendritic cells in forkhead‐box‐P3 (FOXP3)‐deficient scurfy mice lacking functional regulatory T cells. (b) Glycosphingolipids patterns on CD4+ T cells are not affected in absence of FOXP3. (c) Expression patterns and quantities of glycosphingolipids on immune cells could serve as signatures to map inflammation and homeostatic conditions in health or as biomarkers for identifying homeostatic perturbances.