Precision transcriptomics of viral foci reveals the spatial regulation of immune‐signaling genes and identifies RBOHD as an important player in the incompatible interaction between potato virus Y and potato. (1st September 2020)

• Record Type: Journal Article
• Authors: Lukan, Tjaša; Pompe‐Novak, Maruša; Baebler, Špela; Tušek‐Žnidarič, Magda; Kladnik, Aleš; Križnik, Maja; Blejec, Andrej; Zagorščak, Maja; Stare, Katja; Dušak, Barbara; Coll, Anna; Pollmann, Stephan; Morgiewicz, Karolina; Hennig, Jacek; Gruden, Kristina
• Is Part Of: Plant journal. Volume 104:Number 3(2020)
• Page Start: 645
• Page End: 661
• Publication Date: 2020-09-01
• Subjects: immune signaling network -- NADPH oxidase RBOHD -- reactive oxygen species -- salicylic acid -- Solanum tuberosum (potato) -- spatiotemporal response analysis -- virus resistance -- Potyvirus; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.14953
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: Whereas the activation of resistance (R) proteins has been intensively studied, the downstream signaling mechanisms leading to the restriction of the pathogen remain mostly unknown. We studied the immunity network response conditioned by the potato Ny‐1 gene against potato virus Y. We analyzed the processes in the cell death zone and surrounding tissue on the biochemical and gene expression levels in order to reveal the spatiotemporal regulation of the immune response. We show that the transcriptional response in the cell death zone and surrounding tissue is dependent on salicylic acid (SA). For some genes the spatiotemporal regulation is completely lost in the SA‐deficient line, whereas other genes show a different response, indicating multiple connections between hormonal signaling modules. The induction of NADPH oxidase RBOHD expression occurs specifically on the lesion border during the resistance response. In plants with silenced RBOHD, the functionality of the resistance response is perturbed and the spread of the virus is not arrested at the site of infection. RBOHD is required for the spatial accumulation of SA, and conversely RBOHD is under the transcriptional regulation of SA. Using spatially resolved RNA‐seq, we also identified spatial regulation of an UDP‐glucosyltransferase, another component in feedback activation of SA biosynthesis, thus deciphering a novel aspect of resistance signaling. Significance Statement: To progress towards sustainable resistance in the field, it is important to understand the mechanisms of immune signaling, and therefore we studied the regulation of the resistance response of potato against potato virus Y on biochemical, ultrastructural and gene expression levels. We show that SA synthesis and NADPH oxidase RBOHD are interconnected through a regulatory loop that is required for the efficient spatial regulation of the resistance response against potato virus Y in potato.