Respiratory syncytial virus tropism for olfactory sensory neurons in mice. Issue 2 (8th January 2020)

• Record Type: Journal Article
• Authors: Bryche, Bertrand; Frétaud, Maxence; Saint‐Albin Deliot, Audrey; Galloux, Marie; Sedano, Laura; Langevin, Christelle; Descamps, Delphyne; Rameix‐Welti, Marie‐Anne; Eléouët, Jean‐François; Le Goffic, Ronan; Meunier, Nicolas
• Is Part Of: Journal of neurochemistry. Volume 155:Issue 2(2020)
• Page Start: 137
• Page End: 153
• Publication Date: 2020-01-08
• Subjects: nasal cavity -- neurotropism -- odorant binding protein -- olfaction -- olfactory epithelium -- Steno's gland; Neurochemistry -- Periodicals; 616.8042
• DOI: 10.1111/jnc.14936
• Languages: English
• ISSNs: 0022-3042

Abstract

Abstract: The olfactory mucosa, where the first step of odor detection occurs, is a privileged pathway for environmental toxicants and pathogens toward the central nervous system. Indeed, some pathogens can infect olfactory sensory neurons including their axons projecting to the olfactory bulb allowing them to bypass the blood–brain barrier and reach the central nervous system (CNS) through the so‐called olfactory pathway. The respiratory syncytial virus (RSV) is a major respiratory tract pathogen but there is growing evidence that RSV may lead to CNS impairments. However, the mechanisms involved in RSV entering into the CNS have been poorly described. In this study, we wanted to explore the capacity of RSV to reach the CNS via the olfactory pathway and to better characterize RSV cellular tropism in the nasal cavity. We first explored the distribution of RSV infectious sites in the nasal cavity by in vivo bioluminescence imaging and a tissue clearing protocol combined with deep‐tissue imaging and 3D image analyses. This whole tissue characterization was confirmed with immunohistochemistry and molecular biology approaches. Together, our results provide a novel 3D atlas of mouse nasal cavity anatomy and show that RSV can infect olfactory sensory neurons giving access to the central nervous system by entering the olfactory bulb. Cover Image for this issue: doi: 10.1111/jnc.14765 . Abstract : While the respiratory syncytium virus (RSV) is mainly known for its damaged in the respiratory tract, it could also impact the central nervous system. We explored globally the impact of RSV in the mouse nasal cavity and showed that RSV can infect olfactory sensory neurons giving access to the central nervous system by entering the olfactory bulb. Our study also provides a 3D atlas of the nasal cavity in mice giving tools to improve our understanding of virus tropism in the upper respiratory tract. Cover Image for this issue: 10.1111/jnc.14765 .