Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface. Issue 1 (16th February 2018)

• Record Type: Journal Article
• Authors: Lee, Terry D.; Moore, Roger E.; Keen, Denise
• Editors: Coligan, John E.; Dunn, Ben M.; Speicher, David W.; Wingfield, Paul T.
• Is Part Of: Current protocols in protein science. Volume 74:Issue 1(2013)
• Page Start: 16.8.1
• Page End: 16.8.7
• Publication Date: 2018-02-16
• Subjects: mass spectrometry -- nanoelectrospray -- microscale electrospray interface; Proteins -- Laboratory manuals; Proteins; Clinical Laboratory Techniques; Genetic Techniques; Immunologic Techniques; Proteins; Laboratory manuals; 572.6028
• DOI: 10.1002/0471140864.ps1608s74
• Languages: English
• ISSNs: 1934-3655

Abstract

Abstract: Procedures are described for constructing and using a microscale electrospray interface for direct infusion of samples into mass spectrometers. The sensitivity of the nanospray interface is a result of greatly reducing the flow of sample solution while preserving the analyte signal intensity. The described methodology provides a simple and robust way to analyze individual purified peptide and protein samples, i.e., samples that do not require liquid chromatography separation. Curr. Protoc. Protein Sci . 74:16.8.1‐16.8.7. © 2013 by John Wiley & Sons, Inc.