Analysis of colon‐infiltrating γδ T cells in chronic inflammatory bowel disease and in colitis‐associated cancer. Issue 2 (23rd March 2020)

• Record Type: Journal Article
• Authors: Lo Presti, Elena; Mocciaro, Filippo; Mitri, Roberto Di; Corsale, Anna Maria; Di Simone, Marta; Vieni, Salvatore; Scibetta, Nunzia; Unti, Elettra; Dieli, Francesco; Meraviglia, Serena
• Is Part Of: Journal of leukocyte biology. Volume 108:Issue 2(2020)
• Page Start: 749
• Page End: 760
• Publication Date: 2020-03-23
• Subjects: γδ T cells -- IBD -- chronic inflammation -- CAC -- IL‐17 -- colitis; Leucocytes -- Periodicals; Reticulo-endothelial system -- Periodicals; 571.96
• DOI: 10.1002/JLB.5MA0320-201RR
• Languages: English
• ISSNs: 0741-5400

Abstract

Abstract: Inflammatory bowel disease (IBD) remains a global health problem with a significant percentage of patients progressing to chronic inflammation and colitis‐associated cancer (CAC). Whether or not γδ T cells contribute to initiation and maintenance of inflammation in IBD and in the development of CAC is not known. We have evaluated the frequency, phenotype, and functions of γδ T cells among tissue‐infiltrating lymphocytes in healthy donors and IBD and CAC patients. Results show that Vδ1 T cells are the dominant γδ T‐cell population in healthy tissue, whereas Vδ2 T significantly abound in chronic IBD. Vδ2 T cells produce more IFN‐γ, TNF‐α, and IL‐17 than Vδ1 T cells in chronic inflamed IBD. In CAC patients no significant cytokine production was detected in tissue‐resident Vδ1 T cells, but Vδ2 T cells produced remarkable amounts of IFN‐γ and TNF‐α; these data were confirmed by the analysis of an independent cohort of IBD transcriptomes. Moreover, transcriptomes of IBD patients revealed a clear‐cut clusterization of genes related with the maintenance of the inflammatory status. In conclusion, our results demonstrating that Vδ2 T cells have a proinflammatory profile in chronic IBD are suggestive of their participation in IBD and CAC pathogenesis. Abstract : Tissue infiltrating Vδ2 T cells have a pro‐inflammatory profile in IBD and CAC.