Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells. (26th August 2019)

• Record Type: Journal Article
• Authors: Piel, Damien; Bruto, Maxime; James, Adèle; Labreuche, Yannick; Lambert, Christophe; Janicot, Adrian; Chenivesse, Sabine; Petton, Bruno; Wegner, K. Mathias; Stoudmann, Candice; Blokesch, Melanie; Le Roux, Frédérique
• Other Names: Oliver James D. guestEditor.; Destoumieux‐Garzon Delphine guestEditor.; Pruzzo Carla guestEditor.; Baker‐Austin Craig guestEditor.
• Is Part Of: Environmental microbiology. Volume 22:Number 10(2020)
• Page Start: 4198
• Page End: 4211
• Publication Date: 2019-08-26
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.14776
• Languages: English
• ISSNs: 1462-2912

Abstract

Summary: Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae ( V . crassostreae ) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. In this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V . crassostreae, while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V . crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a type 6 secretion system (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5 . 7, are necessary for virulence but act independently to cause haemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V . aestuarianus and V . tapetis, which infect adult oysters and clams respectively. We propose that haemocyte cytotoxicity is a lethality trait shared by a broad range of mollusc pathogens, and we speculate that T6SS was involved in parallel evolution of pathogen for molluscs.