A Rapid Cytologic Double Staining of Epstein-Barr Virus–encoded Small RNA and Cell Surface Markers for Diagnosis of Epstein-Barr Virus–associated Hemophagocytic Lymphohistiocytosis. Issue 8 (November 2020)

• Record Type: Journal Article
• Authors: Takahashi, Natsuki; Kudo, Ko; Tanaka, Miyuki; Kumagai, Naoya; Sato, Tomohiko; Kamio, Takuya; Sasaki, Shinya; Terui, Kiminori; Kurose, Akira; Yanagisawa, Ryu; Nakazawa, Yozo; Ito, Etsuro
• Is Part Of: Journal of pediatric hematology/oncology. Volume 42:Issue 8(2020)
• Publication Date: 2020-11
• Subjects: hemophagocytic lymphohistiocytosis -- Epstein-Barr virus -- Epstein-Barr virus–encoded small RNA -- cytologic diagnosis; Pediatric hematology -- Periodicals; Tumors in children -- Periodicals; 618.9215
• DOI: 10.1097/MPH.0000000000001647
• Languages: English
• ISSNs: 1077-4114

Abstract

Abstract : A 3-year-old boy was clinically diagnosed with Epstein-Barr virus (EBV)-associated hemophagocytic lymphohistiocytosis. We identified EBV-infected CD8-positive T-lymphocytes by cytologic double staining of the peripheral blood for EBV-encoded small RNA and cell surface markers. The patient was subsequently administered immunosuppressive therapy with a reduced dose of etoposide because of previous organ damage. EBV clearance was confirmed by serial quantification of cell-fractionated EBV-DNA, whereas EBV-DNA persisted in the plasma for 18 weeks. Immunochemotherapy with low-dose etoposide combined with serial viral load monitoring is a potential therapeutic option for severe EBV-hemophagocytic lymphohistiocytosis cases with organ damage.